A simple, precise and accurate method was developed for the estimation of amlodipine besylate (AMLO) and irbesartan (IRBE) in synthetic mixture using second order derivative spectrophotometry. Wavelengths selected for quantitation were 233.2 nm for amlodipine besylate (zero crossing point of irbesartan) and 221 nm for irbesartan (zero crossing point of amlodipine besylate). The method was validated with respect to linearity, accuracy, precision, limit of detection and limit of quantitation in accordance with the international conference on harmonization (ICH) guidelines. Linearity was observed in concentration range of 8-48 μg/ml for amlodipine besylate and 2-14 μg/ml for irbesartan. The limit of detection and limit of quantitation were found to be 0.275 μg/ml and 0.883 μg/ml for amlodipine besylate and 0.126 μg/ml and 0.384 μg/ml for irbesartan. The percentage recovery of amlodipine besylate and irbrsartan was found to be 99.53% and 99.39%, respectively. The % R.S.D. values for intra-day and inter-day precision study were <2.0%, confirming that the method was precise. The method can be successfully employed for the simultaneous estimation of amlodipine besylate and irbesartan in synthetic mixture.
Loading....